recombinant mouse cxcl2 protein  (r&d systems)

Journal: EMBO Reports

Article Title: CMTM3 regulates neutrophil activation and aggravates sepsis through TLR4 signaling

doi: 10.1038/s44319-024-00291-7

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Recombinant Mouse CXCL2 Protein , R&D Systems , 452-M2.

Techniques: Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Red Blood Cell Lysis, cDNA Synthesis, SYBR Green Assay, Real-time Polymerase Chain Reaction, Isolation, Membrane, Software

Journal: Aging (Albany NY)

Article Title: PDCD10 promotes the aggressive behaviors of pituitary adenomas by up-regulating CXCR2 and activating downstream AKT/ERK signaling

doi: 10.18632/aging.204206

Figure Lengend Snippet: Activation of CXCR2 rescues the inactivation of AKT/ERK signaling and the tumor-suppressive effects induced by PDCD10 silencing. ( A , B ) Western blotting was performed to examine the phosphorylation levels of AKT and ERK1/2 after recombinant CXCL2 administration (200ng/ml) in Att-20 and TtT/GF cells with PDCD10 silencing. ( C , D ) CCK-8 assay was used to evaluate cell proliferation potential after CXCL2 administration in Att-20 and TtT/GF cells with PDCD10 silencing. ( E , F ) Relative migration rate of ATT-20 and TtT/GF cells with PDCD10 silencing was analyzed by scratch assay after CXCL2 administration (magnification: 100x). ( G , H ) Transwell invasion assay was employed to assess the invasion capacity of Att-20 and TtT/GF cells with PDCD10 silencing after CXCL2 administration (magnification: 200x). * P < 0.05.

Article Snippet: Recombinant mouse CXCL2 protein (R&D System) was added into the culture medium to study the signaling pathways in a subset of experiments as indicated.

Techniques: Activation Assay, Western Blot, Phospho-proteomics, Recombinant, CCK-8 Assay, Migration, Wound Healing Assay, Transwell Invasion Assay